Autofluorescence analysis by deconvolution microscopy. (A) Small cell with few blue autofluorescent structures at the start of culture. (B) Same cell as panel A, by phase-contrast microscopy. (C) Intermediate size cell with many, paranuclear, blue autofluorescent structures after 7-day culture with TGF-β. (D) Same cell as panel C by phase-contrast microscopy: the cell is dendritic in shape. (E) Large cells with many autofluorescent structures that appear in part green, after 14-day culture with TGF-β. (F) Same cells as panel E by phase-contrast microscopy: the cells are markedly dendritic in shape. (G-H) Cell after 18-day culture with TGF-β; green autofluorescent structures are less numerous and less intensely fluorescent than after 14-day culture (compare with panel E). Bar represents 10 μm. (I-J) Spectra of autofluorescence emission after 1-day (black), 7-day (dark gray), 14-day (continuous light gray), and 18-day culture (discontinuous light gray). (J) The spectra were normalized assuming the respective highest peak as 100%. Live cells were mounted on slides with PBS; images were taken with ViSTA 1.9.3 software through a KAF261E detector (Kodak) inserted in a Hires IV camera (DTA) applied to an Eclipse TE-2000-E microscope with a 100×/1.3 NA oil objective (Nikon), and were processed for print with Photoshop 6.0 for Macintosh (Adobe).