Flow cytometry. (Top row) Percentages of cells expressing several membrane antigens after 7- and 14-day culture with TGF-β (mean and range). Only CD133, CD34, and CD45 were tested at the start of culture. The differences for CD133 and CD34 were significant (P < .05). For CD14 and CD16, the scale is limited to 2% because these antigens were expressed by a strict minority of cells. Far right: Results of a representative experiment showing the expression of CD207/langerin (Dendritics) after 18-day culture with TGF-β. (Middle row) Results of a representative experiment showing the expression of MHC-II and of late differentiation markers of dendritic cells after 18-day culture with TGF-β. (Bottom row) Results of a representative experiment showing the expression of MHC-II and of late differentiation markers of dendritic cells after 18-day culture without TGF-β.