Figure 5
Electron microscopy details. (A-F) Details of cells cultured with TGF-β. (A-C) Variably well-developed multilaminar inclusions (14-day culture). (D) Smooth endoplasmic reticulum (18-day culture). (E) Myelin-like figures (18-day culture). (F) Tubuloreticular inclusions (14-day culture). Bars represent 100 nm. (G-H) Percentage of cells in apoptosis (G) and in necrosis (H) in cultures with TGF-β (black column) and without TGF-β (gray column); only the difference in apoptosis at 14 days was significant (P < .05; χ2 test). Images were taken with analySIS 5.0 software through a MegaView III camera applied to a JEM 1010 electron microscope at 80kV (Jeol), and were processed for print with Photoshop 6.0 for Macintosh (Adobe).
