Immunofluorescent labeling. (Left panels) Percentage of labeled cells at immunocytochemistry at 7, 14, and 18 days of culture with (black columns) and without TGF-β (gray columns; mean ± SD). (Middle panels) Immunolabeled cells at fluorescence microscopy, on 18-day culture with TGF-β. (Right panels) Phase-contrast microscopy of the same fields as in the middle panels. (A-C) MHC-II: all the differences between TGF-β treated and untreated cultures were significant (P < .05). (D-F) CD1a; the difference at 18 days was significant (P < .05). (G-I) CD207/langerin (antibody from Novocastra Laboratories). (J-L) CD207/langerin (antibody from Dendritics); note that cellular debris were not stained (arrows). (M-O) CD54; note that cellular debris were not stained (arrows). Bar represents 20 μm. Slides were mounted with Gel/Mount (Biomeda), images were taken with Axio Vision 4.1 software through an AxioCam HRm camera applied to an Axioskop microscope with a 40×0.75 NA air objective (Carl Zeiss), and were processed for print with Photoshop 6.0 for Macintosh (Adobe).