Jagged2 is abundantly expressed by human TECs. (A) Quantitative RT-PCR analysis of human TECs for the expression of Notch ligands, DLL1, DLL4, JAG1, and JAG2. Expression levels are normalized using the ΔΔC_t method, relative to GAPDH and relative to levels in total thymus. Results show mean of 3 independent sets of samples. Errors bars represent SEM. (B) Flow cytometric analysis of Delta-like-1, Delta-like-4, and Jagged2 expression in human cTECs (top row) and mTECs (bottom row). Histogram plots show isotype control (black) versus specific Notch ligand antibody staining (gray). Data are representative for at least 2 independent donors.