Expression of the truncated form of ANXA2. A total of 10 μg of A549 cell extract (lane 1), 200 ng of plasmin proteolyzed, N-terminal truncated ANXA2 (Ala-28–Asp-338, lane 2), or 10 μg of cell extract from BL21 cells expressing ANXA2 (Ser-1–Lys-307, lane 3) was subjected to 10% or 12% SDS-PAGE as indicated. Proteins were transferred onto nitrocellulose membrane and analyzed by Western blotting with the antibodies indicated. The data show that the WT ANXA2 (Ser-1–Asp-338) is easily resolved from the putative plasminogen-binding, C-terminal truncated ANXA2 (Ser-1–Lys-307).