American Society of Hematology

Figure 1

$Figure 1. Characterization of SP heterogeneity in the panel of MM cell lines. (A) Representative flow cytometric dot plots of SP analysis. Dot plots show control OPM1 cells incubated in Hoechst 33342 alone (left), Hoechst 33342 accumulation in the presence of 100μM verapamil (middle), and Hoechst 33342 accumulation in the presence of 50μM reserpine (right). Abscissa is Hoechst red fluorescence intensity and ordinate is Hoechst blue fluorescence intensity, with gate representing the SP fraction of OPM1 cells. (B) Quantification of SP fraction at 1 day in MM cell lines alone and in the presence of 100μM verapamil or 50μM reserpine. (C) Percentage of SP fraction in 11 primary samples of patient with MM.$

Characterization of SP heterogeneity in the panel of MM cell lines. (A) Representative flow cytometric dot plots of SP analysis. Dot plots show control OPM1 cells incubated in Hoechst 33342 alone (left), Hoechst 33342 accumulation in the presence of 100μM verapamil (middle), and Hoechst 33342 accumulation in the presence of 50μM reserpine (right). Abscissa is Hoechst red fluorescence intensity and ordinate is Hoechst blue fluorescence intensity, with gate representing the SP fraction of OPM1 cells. (B) Quantification of SP fraction at 1 day in MM cell lines alone and in the presence of 100μM verapamil or 50μM reserpine. (C) Percentage of SP fraction in 11 primary samples of patient with MM.

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