Ang-2 induced skin infiltration of macrophages. Immunostaining of WT and Ang-2 transgenic skin sections using various leukocyte markers. Representative images after 52 weeks of transgene expression are displayed, and the number of CD45⁺, F4/80⁺, Ly-6G⁺, CD4⁺, and CD45R⁺ B cells per millimeter squared were determined. CD45⁺ leukocytes accumulate progressively on prolonged Ang-2 expression (A). Among these, F4/80⁺ macrophages (B) and Ly-6G⁺ neutrophil granulocytes (C) are enriched, whereas CD4⁺ T cells and CD45R⁺ B cells compose only a minor fraction (indicated by arrowheads) that remains constant over time (C-D). Data are mean ± SEM of 5 animals. P values represent significance over WT cell count. Scale bar represents 100 μm. *P < .05. **P < .01. ***P < .005.