Total cell lysates and subcellular proteome extraction of HUVECs stimulated with TNFα. (A) Representative Western blot of total cell lysates stimulated with TNFα and IL-8 and densitometry. HUVEC monolayers were stimulated for 30 minutes or 4 hours with IL-8, or for 4 hours with TNFα. (B) Rrepresentative Western blot of subcellular proteomic extraction. HUVEC monolayers were stimulated and treated with or without LMB (nuclear export inhibitor). Fractions were blotted for LSP1 (arrows), pan-cadherin (membrane marker), ATF2 (nucleus), and actin (cytoskeleton). Densitometry of the Western blots in panel A (n = 3) is relative to total actin (as standardization). Error bars indicate SEM. *P < .05.