Serial sections from micrographs of migrating neutrophils in WT and Lsp1^−/− mice. Sections were taken after KC superfusion (5nM) of the cremaster. Serial sections (each 70 nm) were cut 350 nm apart. Filled arrows demonstrate endothelial dome formations in WT mice (A-D), and open arrows point the lack of such formations in LSP1-deficient mice (E-H). Scale bar indicates 500 nm. e indicates endothelial cell; n, neutrophil; and L, vessel lumen. Image acquisition: Hitachi H-7000 transmission electron microscope; direct magnification: 6000×; 16000 AMT camera, AMT Capture Engine software (V.600.128); images generated with Microsoft Office PowerPoint 2003 (SP3).