GO-203 increases superoxide (O2−) and hydrogen peroxide (H2O2) levels in multiple myeloma cells. (A-D) U266 (A,C), and RPMI8226 (B,D) cells were left untreated, and treated with 5μM GO-203 or 5μM CP-2 each day for 2 days. (A-B) The cells were incubated with hydroethidine for 30 minutes. Fluorescence of oxidized hydroethidine was determined by flow cytometry (left). The results are expressed as the relative superoxide level (mean ± SD of 3 determinations) compared with that obtained for untreated cells (right). (C-D) The U266 (C) and RPMI8226 (D) cells were incubated with c-H2DCFDA for 30 minutes. Fluorescence of oxidized DCF was measured by flow cytometry (left). The results are expressed as the relative hydrogen peroxide level (mean ± SD of 3 determinations) compared with that obtained for control cells (right).
