TIGAR expression is decreased by ROS. (A-B) U266 (A) and RPMI8226 (B) cells were left untreated, and treated with 5μM GO-203 or 5μM CP-2 each day for 3 days. The GO-203–treated cells were also incubated in the presence of 5mM NAC for the last 2 days. Lysates were immunoblotted with the indicated antibodies. (C-D) U266 (C) and RPMI8226 (D) cells were treated with 250μM H₂O₂ for the indicated times. Lysates were immunoblotted with the indicated antibodies.