Nonhematopoietic differentiation of MPS-iPS cells. Same histologic section of mature teratoma from immunodeficient mouse injected with MPS-iPS cells (top, magnification 4×) shows melanocytes of ectodermal origin (bottom left arrows), osteoid deposition and osteocytes of medodermal origin (bottom middle arrows), columnar epithelium of endodermal origin (bottom right; goblet cells are indicated by arrows; magnification 20×). Similar mature teratomas with contribution of ectodermal, mesodermal, and endodermal-derived cells formed after injection of early (< 20) passage and late (> 20) passage iPS cells from wild-type keratinocytes and MSCs (n = 11), from patient 1 (keratinocyte-derived iPS cells, n = 3; IDUA-corrected keratinocyte-derived iPS cells, n = 3) and from patient 2 (keratinocyte-derived iPS cells, n = 2; MSC-derived iPS cells, n = 2; data not shown). Hematoxylin-eosin stain. Images were obtained with an Olympus BX51 Microscope, magnification 4×/0.16 and 20×/0.70. Spot RT camera and Spot RT software v3.2 were used in acquiring the images. All images were taken at room temperature.