CDPs give rise to increased numbers of DCs in inflamed LNs. CD45.1xCD45.2 F1 hosts were transplanted with freshly isolated, CFSE-labeled CD45.1⁺ CDPs and injected with either 25 μg of CpG or PBS into one foot pad. The plots show CD19⁻ NK1.1⁻ cells on day 4 in LNs draining the injected side and the noninjected side of a representative CpG-treated animal (A). The cells were first gated CFSE⁺ to separate transferred CDP-derived cells from the host background (left) and further gated CD45.1⁺ CD45.2⁻ cells (data not shown). The gating for pDCs (CD11c^low CD45RA⁺), CD11c^int MHC II^hi, and CD11c^hi MHC II^int DCs derived from transferred CDPs is indicated (middle to right). The bar graph in panel B shows the average number of CDP-derived cells in the LNs from the injected and the noninjected side of animals treated with CpG or PBS. The numbers drawn on the graph indicate the average fold increase of CDP-derived DCs on the injected compared with the noninjected side. The number of total CDP-derived cells in the BM of PBS- and CpG-treated animals it shown in panel C. The graphs show the means and SEM of 4 independent experiments, each using 1 CpG- and 1 PBS-treated recipient mouse, and the level of significant difference between inflamed and noninflamed organs is indicated.