Raman spectroscopy analysis of the cortical bone tissue of WT, oim, and oim+IUT mice. (A) PC1 versus PC2 obtained demonstrates that PC1 differentiates WT from oim and oim+IUT. (B) Weighting of spectrum frequencies contributing to PC1. (C) Subset of data from panel A showing that female oim and oim+IUT are differentiated by PC2. (D) Weighting of spectrum frequencies contributing to PC2. (E) Carbonate content. (F) Apatite crystallinity obtained by univariate peak analysis of the Raman spectra. *P < .05 (significant difference vs WT group). †P < .05 (significant difference vs oim group).