TPO-induced signaling is enhanced in Cib1^−/− megakaryocytes. (A) Western blots of lysates from untreated or TPO-treated WT and Cib1^−/− BM-derived megakaryocytes purified using a discontinuous BSA gradient. Membranes were initially probed for phosphorylated Akt^T308 and phosphorylated ERK1/2, then stripped and reprobed for total Akt or total ERK1/2, respectively. (B) Quantification of band intensity from panel A normalized to untreated WT sample. *P < .05 vs WT + TPO (n = 5). (C) Western blots of lysates from WT and Cib1^−/− megakaryocytes treated with or without TPO. Membranes were probed for phosphorylated FAK^Y925, then stripped and reprobed for total FAK. (D) Quantification of band intensity from panel B, normalized to untreated WT sample. *P < .05 vs WT + TPO (n = 5).