Cib1 deletion causes inhibition of megakaryocyte migration and attachment to ECM proteins. (A) Percentage of WT and Cib1^−/− megakaryocytes attached to BSA, Fg, or Fn. *P < .05 vs WT (n = 6). (B) Diff-Quik stained membranes from transwell migration assays using WT and Cib1^−/− megakaryocytes. Arrows point to megakaryocytes. Images were captured using a Nikon Eclipse TS100 microscope: top (original magnification ×40) and bottom (original magnification ×200). (C) Quantification of megakaryocytes per well after transwell migration assays. *P < .05 vs WT (n = 7). (D) Phase-contrast images of BM-derived megakaryocytes purified using discontinuous BSA gradient from WT and Cib1^−/− mice attached for 5 hours to immobilized Fg. The arrow indicates a proplatelet-producing megakaryocyte. Images were captured using a Zeiss Axiovert microscope (original magnification ×200). (E) Quantification of the percentage of proplatelet-producing megakaryocytes derived from WT and Cib1^−/− mice. *P < .05 vs WT (n = 6).