GVL activity is largely preserved in RORγt^−/−/T-bet^−/− T cells. Lethally irradiated BALB/c mice received TCD-BM cells alone or plus 2 × 10⁶ naive T cells from WT or RORγt^−/−/T-bet^−/− donors. Recipients were given 2 × 10³ A20 tumor cells with luciferase transgene at the same time of transplantation. Recipient survival (A) and body weight changes (B) are shown. (C) Tumor growth in recipients was monitored with in vivo bioluminescent imaging. Data shown were combined from 2 replicate experiments with 8 mice in each group. (D-E) Lethally irradiated BALB/c mice received TCD-BM cells alone or plus 5 × 10⁵ naive T cells from WT or RORγt^−/−/T-bet^−/− donors. Recipients were given 2 × 10³, 1 × 10⁴ or 5 × 10⁴ A20 tumor cells with luciferase transgene at the same time of transplantation. The summary of BLI signal intensity in WT (D) or RORγt^−/−/T-bet^−/− (E) recipients are shown at multiple time points after BMT. Six recipients were included in all the groups, except 5 in the TCD-BM alone group. Asterisk indicates statistical significance between WT and RORγt^−/−/T-bet^−/− recipients; ***P < .001.