Impact of Pknox1-MC, Meis1, and Pknox1 expression on global gene expression profile of Hoxa9-transduced cells. (A) Left panel: Survival plot of mice transplanted with 2 × 105 cells used for expression profiling. The genotypes of the transplanted cell populations are shown on top. Right panel: Western blot analysis of FLAG-tagged protein expression in lysates from recovered cells. Tubulin-α levels are shown as a loading control. (B) Venn diagram depicting strategy for identification of genes exhibiting in response to Meis1 (group I), Pknox1 (group II), and Pknox1-MC (group III) at least 2-fold increase in expression levels compared to the parental Hoxa9 cell population. The number of genes found in each of these datasets is indicated inside the circle. (C) Identification of transcript clusters enriched in GO terms associated with distinct biological processes from the gene datasets shown in panel B, as determined by the DAVID Version 6.7 bioinformatics software. Only the top 2 enriched GO terms are shown for each functional cluster. The Enrichment Score refers to the negative log transformation on the geometric mean of the P values obtained for each of the clustered enriched term. The Benjamini-corrected P value provided for each biologic process represents a 1-tailed Fisher Exact Probability Value further corrected for false discovery rate using the Benjamini correction.
