PBMCs from lymphoma patients have decreased functions mediated by monocytes. (A) PBMCs from controls (n = 6) and lymphoma patients (n = 6) were incubated with influenza vaccine, and their cytokine expressions were measured by ELISpot assay for IFN-γ_, IL-4, and IL-17 production. Each column represents mean plus or minus SD of 6 samples. (B) Monocytes from controls (n = 8) and lymphoma patients (n = 8) were cocultured for 3 days with negatively selected T cells pooled from 3 allogeneic healthy donors. Proliferation was assessed by H³ incorporation over the last 18 hours. (C) PBMCs and PBMCs with monocytes removed were cultured for 3 days with anti-CD3/CD28 beads. Proliferation was assessed by H³ incorporation over the last 18 hours (controls, n = 6; lymphoma, n = 13.) Statistically significant P value is shown between groups. NS indicates that P value was not significant.