NET formation in response to C albicans is impaired in MPO-deficient neutrophils. Neutrophils were co-incubated with the yeast form of C albicans at an MOI of 10, or activated with PMA, for 2 hours, and DNA was stained with Sytox. (A-D) Images of Sytox fluorescence. (E-F) Quantitation of nuclear areas. Neutrophils from a healthy donor formed NETs upon stimulation with C albicans (A,E) and PMA (C,F), but neutrophils from a completely MPO-deficient donor (donor 1) did not form NETs in response to C albicans (B,E) or PMA (D,F). Scale bar, 25 μm.