Inhibition of ARC promotes apoptosis and sensitizes Ara-C-induced cell death in AML cells. (A) OCI-AML3 cells were treated with ARC ASO by electroporation for 72 hours. ARC protein levels were determined at 48 hours by Western blot analysis. (B) OCI-AML3 cells were treated with ARC ASO (1 μg/million cells) for 24 hours, then with Ara-C for an additional 48 hours. Cell death was determined by annexin V (AnnV) staining in the presence of 7-amino-actinomycin D (7AAD).