CX3CR1-GFP expression on different NK-cell subsets. Cells from BM, spleen (Sp), and liver (Liv) from 6-10 week old CX3CR1^+/GFP mice were isolated. Immunofluorescence staining with anti-NK1.1– and anti-CD3ϵ–specific mAbs, in combination with anti-CD11b– and anti-KLRG1–specific mAbs was performed, and expression of CX3CR1-GFP was analyzed on CD3⁻/NK1.1⁺ NK cells (A), and on the different NK-cell subsets (B): CD11b^low/KLRG1⁻ cells (population 1), CD11b^high/KLRG1⁻ cells (population 2) and CD11b^high/KLRG1⁻ cells (population 3). Numbers in the histogram plots indicate the percentage of positive cells of a representative experiment of at least 3 performed. In the bottom panels, the mean values ± SD of the percentage of CX3CR1-GFP⁺ NK cells in the different populations from the organs of a total of 9 animals analyzed in independent experiments, are represented.