KLRG1⁺/CX3CR1-GFP⁺ NK cells derive from the KLRG1⁺/CX3CR1-GFP⁻ counterpart. Sorted KLRG1⁺/CX3CR1-GFP⁻ and KLRG1⁺/CX3CR1-GFP⁺ NK-cell populations (purity > 95%) from the spleen of heterozygous CX3CR1^+/GFP mice (CD45.2) were adoptively transferred into wt C57BL/6 mice (CD45.1). After 12 days, donor NK cells from BM, spleen, and liver were identified as CD45.1⁻/CD45.2⁺/CD3⁻/NK1.1⁺ cells and analyzed for their expression of KLRG1 and GFP. Numbers in plots indicate the mean percentage ± SD of CX3CR1-GFP⁺ donor NK cells in the recipient spleen. Data are representative of 1 of at least 3 experiments performed.