Assessment of plasma cytokine levels in L1Cre(−)Bmpr2^f/f and L1Cre(−)Bmpr2^f/f mice treated with SCH527123 or vehicle. Plasma from L1Cre(−)Bmpr2^f/f mice or L1Cre(+)Bmpr2^f/f mice treated with SCH527123 or vehicle was assessed for levels of multiple proinflammatory cytokines using a commercial proteome profile array (A-B). The levels of each cytokine could be observed as a pair of duplicate spots (A) and semiquantified by subtracting the background pixel density from the total pixel density of each duplicate spot and represented as the average of both pixel densities for each pair of spots (B). (B) Hatched bars represent L1Cre(+)Bmpr2^f/f mice treated with vehicle; and open bars, L1Cre(+)Bmpr2^f/f mice treated with SCH527123. KC levels in pooled plasma from each group of mice were then quantified by commercial ELISA (C). Data are the mean ± SEM for a minimum of 8 animals per group. ***P < .001, compared with L1Cre(−)Bmpr2^f/f mice by Dunnett test. **P < .01, compared with vehicle-treated L1Cre(+)Bmpr2^f/f mice by Dunnett test.