Cdc42 is required for HIV-1 transfer from DCs to autologous resting CD4⁺ T cells in the presence of sAg. (A) HIV-1 infection transfer to resting autologous CD4⁺ T lymphocytes after sAg stimulation. (B) HIV-1 infection transfer in DC-resting autologous CD4⁺ T lymphocyte cocultures. DC/T ratio 5:1 (left panel) and DC/T ratio 1:5 are shown (right panel). Data are mean ± SD of 3 independent experiments. (C) Fold decrease of HIV-1 transfer in DCs: resting autologous CD4⁺ T lymphocyte ratio conditions 5:1 (left panel) and 1:5 (right panel). Data are mean ± SD of 3 independent experiments. (D) Impact of Cdc42 or Larg silencing in MyDCs on HIV-1 transfer to Jurkat CD4⁺ T lymphocytes (left panel). Data are mean ± SD of 4 independent experiments. Two representative images of MyDCs-CD4⁺ T cell infectious synapses are shown (middle and right panels). Bar represents 5 μm. *P < .05 (Student t test). Bonferroni test with an α-error value of 5% has been applied to all panels with multiple comparisons.