Nab2 blocks TRAIL induction in restimulated helpless CD8⁺ T cells. CD8⁺ T cells were purified from TAP^−/−Ad5E1-MEC–primed, helpless mice and were transfected with 2 μg of pcDNA3.1-Nab2 or the empty control vector, together with 0.5 μg of the reporter plasmid pMAX-GFP. (A) Twenty-four hours after transfection, GFP expression was determined as a measurement for transfection efficiency. (B-D) CD8⁺ T cells were cultured for 4 hours in the presence or absence of the E1B_192-200 peptide, and the relative expression of TRAIL (B) and IFNγ (D) mRNA was determined. (C) The fold induction of TRAIL in Nab2- or EV-transfected CD8⁺ T cells was assessed by comparing mRNA levels before and after E1B_192-200 peptide restimulation. Each connected dataset represents an independently performed experiment (n = 4; T cells were pooled from 4 mice per group). *P < .05; ***P < .005.