Characterization of gene-corrected SCD iPSC clones. Gene corrected SCD iPSC clones (c36 and cre4 shown here) display characteristic pluripotency markers such as alkaline phosphatase (AP), OCT4, NANOG, and TRA-1-60 (A); maintain normal karyotypes (B); and form teratomas bearing cells from all 3 germ layers, that is, ectoderm, mesoderm, and endoderm (C).