Hoxa3 promotes proliferation and differentiation of HSC/Ps into Gr-1⁺CD11b⁺ myeloid cells. (A) Viable cell number at weekly time points following Hoxa3-mCherry or mCherry protein delivery to HSC/Ps. (B) Total colony forming units (CFU) at weekly time points following Hoxa3-mCherry or mCherry protein delivery to HSC/Ps. (C-D) The percentage of CFU-monocytic (C), and CFU-granulocytic (D), at weekly time points following Hoxa3-mCherry or mCherry protein delivery to HSC/Ps (error bars represent SEM, *P ≤ .05, **P ≤ .01, ***P ≤ .001). (E-F) qRT-PCR analyses of lineage-specific gene expression at weekly time points following Hoxa3-mCherry or mCherry protein delivery to HSC/Ps. Gr-1/Ly6g is a granulocytic marker, and CD11b/Itgam is a pan-myeloid marker.