Inflammation marker expression in the skin during acute inflammation. (A-B) Real-time RT-PCR analyses were performed using RNAs from whole ear and back skin harvested 2 days after oxazolone challenge (2-day oxa; n = 6-10 per group) or UVB irradiation (2-day UVB; n = 5-8 per group) and from skin of untreated K14-VEGF-C, K14-VEGF-D Tg, and wild-type mice (n = 3-5 per group). All inflammation markers shown were significantly up-regulated in K14-VEGF-C, K14-VEGF-D Tg, and wild-type mouse ear (A) and back skin (B) 2 days after oxazolone challenge (A) or UVB irradiation (B), compared with untreated wild-type or transgenic mice. S100A8, S100A9, and CXCL2 mRNA levels were slightly increased in K14-VEGF-C Tg mice 2 days after UVB irradiation compared with irradiated wild-type mice (B). (C) ELISA analyses of ear lysates showed significantly increased levels of IL-1β, VEGF-A, and CCL2 at 2 days after oxazolone challenge or UVB irradiation in the ear skin of K14-VEGF-C, K14-VEGF-D Tg, and wild-type mice compared with untreated mice. The protein levels of IL-1β and VEGF-A were slightly but significantly reduced in the ear skin of oxazolone challenged K14-VEGF-C Tg mice compared with oxazolone challenged wild-type mice. (A-C) Data are mean ± SD. *P ≤ .05 versus oxazolone challenged or UVB irradiated wild-type mice. **P ≤ .01 versus oxazolone challenged or UVB irradiated wild-type mice.