Defective thrombus formation in the absence of Pyk2 or catalytically active PI3Kβ. CSFE-labeled platelets in whole blood from wild-type (WT), Pyk2 KO, and PI3KβKD mice were perfused over immobilized monomeric collagen at a shear rate of 1000/s for 4 minutes. Images were taken after brief rinse of the coverslips with washing buffer (2 minutes) and are reported in the top rows (A). Thrombus formation on the coverslips was evaluated by measuring the covered area in 10 different and randomly taken microscopic fields and results are reported in the histogram in the bottom row (B, black bars) as the means ± SD of 4 different experiments. Coverslips were then further perfused with washing buffer for 10 minutes, and additional images were taken to evaluate thrombus stability. The remaining area covered by thrombi on extensive washing is reported in the histogram in the bottom row (B, white bars) as the means ± SD of 4 different experiments.
