Grp78 heterozygosity suppresses PTEN null-mediated MPD characteristics. All analyses were performed 6 days after completion of pIpC treatment. (A) Organ size and morphology from mice of the indicated genotypes. (B) H&E staining of paraffin sections of spleen (top) and liver (bottom) of the indicated genotypes. The scale bar represents 200 μm. Sections of spleen and liver were mounted with Pro-Texx mounting medium (Lerner Laboratories, #137635) and stained with H&E. Images were acquired with a Leica Type DMLB2 microscope (10×/0.3 objective) fitted with SPOT RT KE SE Model 7.3 Three Shot Color camera and SPOT 4.0.5 computer software (Diagnostic Instruments Inc). (C) Quantitation of the spleen weight (n = 8), total BM (BM) cell number (n = 8), and percentage of LSK cells in BM (n = 5) for each indicated genotype. (D) Quantitation of cell cycle distribution of LSK cells from WT (n = 3), cP^f/f (n = 5), and cP^f/f78^f/+ (n = 5). (E) Flow cytometric analysis of apoptotic LSK cells using annexin V and 7-AAD. Data are as mean ± SE. *P < .05 (Student t test). **P < .01 (Student t test). ***P < .001 (Student t test).