Pten null Grp78 heterozygous mice show MPD characteristics after longer latency period. All analyses were performed 21 days after completion of pIpC treatment. (A) Organ size and morphology from mice of the indicated genotypes. (B) H&E staining of paraffin sections of spleen (top) and liver (bottom) of the indicated genotypes. Scale bar represents 200 μm. Sections of spleen and liver were mounted with Pro-Texx mounting medium (Lerner Laboratories, #137635) and stained with H&E. Images were acquired with a Leica Type DMLB2 microscope (10×/0.3 objective) fitted with SPOT RT KE SE Model 7.3 Three Shot Color camera and SPOT 4.0.5 computer software (Diagnostic Instruments Inc). (C) Quantitation of the spleen weight (n = 6), total BM cell number (n = 6), and percentage of LSK cells in BM (n = 5) and percentage blast cells in BM (n = 6) for each indicated genotype. (D) Complete blood count with tail peripheral blood from WT (n = 7), cP^f/f (n = 7), and cP^f/f78^f/+ (n = 7) mice. Peripheral blood was collected via tail bleeding and analyzed using an auto hematology analyzer BC-2800 vet (Mindray) according to the manufacturer's instructions. Data are mean ± SE. *P < .05 (Student t test). **P < .01 (Student t test). ***P < .001 (Student t test).