Naive-derived effector cells demonstrate resistance to terminal differentiation. Cryopreserved leukapheresis samples were thawed and rested overnight. CD8⁺ T-cell subsets were isolated then stimulated with allogeneic feeders, anti-CD3, and IL-2. Cells were transduced to express the 1G4-α-95:LY mutant TCR 48 hours after stimulation. The following data were obtained 10-16 days after stimulation. (A) KLRG1 expression by real time RT-PCR. Expression is relative to ACTB then normalized to the average relative expression for each patient. Data from 5 patients are shown. (B) Flow cytometric analysis of CD57 expression. Gate frequencies are indicated. Data shown are representative of 3 patients.