Naive-derived effector cells display indicators of enhanced proliferative potential and efficacy. Cryopreserved leukapheresis samples were thawed and rested overnight. CD8⁺ T-cell subsets were isolated then stimulated with allogeneic feeders, anti-CD3, and IL-2. Cells were transduced to express the 1G4-α-95:LY mutant TCR 48 hours after stimulation. The following data were obtained 10-16 days after stimulation. (A) Expression of CD27 as determined by flow cytometry. Gate frequencies are shown. Data are representative of 6 patients. (B) Telomere length relative to average length for each patient. Four individual patients are represented by different shapes (*P < .05).