Mixed leukocyte culture (MLC) and IL-32 expression. (A) PBMCs were cultured for 7 days, and Western blots were generated from either unsorted or sorted CD8⁺ responder cells; 1 blot is representative of 3 similar experiments. (B) IL-32 mRNA levels in allogeneic MLCs. Error bars represent mean ± SEM of 3 similar experiments. Cytokines from MLC supernatants were determined by ELISA, including TNF-α (C), IL-6 (D), and IL-8 (E). Solid columns represent results in allogeneic cultures, open columns represent results in autologous controls. The results are displayed as ± SEM from 3 similar experiments (*P < .01; Student t test).