Inhibition of proliferation and TNFα secretion in MLCs by AAT. (A) Western blot of IL-32β and γ levels in CD8⁺ cells from 7-day MLCs under control conditions and in the presence of AAT (0.3 mg/mL). IL-32 β and γ isoforms in the presence of AAT. The Western blot is representative of 3 similar experiments. (B) Expression changes in IL-32 protein levels in allogeneic MLCs and autologous controls as determined by densitometry (OD) of the same biologic experiment. Open columns reflect results in the absence of AAT; solid columns in the presence of AAT. (C) Proliferation in MLCs (as measured by ³H thymidine uptake; CPM, mean ± SEM). (D) TNF-α ELISA. Secretion of TNF α in the presence and absence of AAT (*P < .05; Student t test).