Effect of AAT on cytokine RNA and protein expression in PBMCs and plasma after transplantation. (A) IL-1Ra, IL-1β, TNF-α, and PR3 RNA levels, determined by RT-PCR, in PBMCs. Levels in AAT-treated mice (n = 6) are expressed relative to levels in albumin treated controls; mean ± SEM (n = 6; log2). (B-D) Mean ± SEM cytokine plasma levels at 3, 7, and 10 days after transplantation. Shown is a panel selected from a mouse array of 40 cytokines, showing significant changes. Changes in cytokine concentration are expressed as percent change compared with albumin control. The horizontal dotted line indicates an increase/decrease of 25%.