Absence of chronic GCs in the gut-associated lymphoid tissues of Dicer1^fl/flAicda^Cre/+ mice. Flow cytometric analysis of GC B cells in the Peyer patches (PP; A), or mesenteric lymph nodes (mLN; B), of unchallenged Dicer1^+/+Aicda^Cre/+ and Dicer1^fl/flAicda^Cre/+ mice. GC B cells were stained as described in Figure 3A. Numbers adjacent to outlined area indicated percentage of cells in the individual gate. (C) Confocal microscopy of PP cryosections from unchallenged Dicer1^+/+Aicda^Cre/+ and Dicer1^fl/flAicda^Cre/+ mice. The sections were stained with the same cocktail of antibodies as described in Figure 3B to visualize B-cell follicles, FDCs, and GC B cells. Data are representative of more than 6 experiments (A-B) or 3 experiments (C).