Figure 5
Figure 5. Immunohistochemistry staining of tissues with 19E3 anti-CAR Ab. (A) Trafficking of targeted T cells to tumor sites in CLL-4 44 hours after T-cell infusion. Images were acquired at room temperature using Nikon 90i Eclipse microscope system (Nikon) with a Nikon Plan Apo VC 20×/0.75 objective lens (magnification 200×), Nikon DigiSight Digital Camera Head and Nikon NSI-Elements Version 3.10 software. (B) Trafficking and homing of targeted T cells to BM. IHC staining on BM biopsies shows the presence of 19-28z+ T cells 5 and 6 weeks after infusion in patients ALL-1 and CLL-7, respectively (C) Trafficking and homing of targeted T cells to BM ≤ 8 weeks after infusion. ND indicates not done; (−) no positive 19-28z+ T cells found; (+) positive 19-28z+ T cells found occasionally, and (++) positive 19-28z+ T cells readily identifiable.

Immunohistochemistry staining of tissues with 19E3 anti-CAR Ab. (A) Trafficking of targeted T cells to tumor sites in CLL-4 44 hours after T-cell infusion. Images were acquired at room temperature using Nikon 90i Eclipse microscope system (Nikon) with a Nikon Plan Apo VC 20×/0.75 objective lens (magnification 200×), Nikon DigiSight Digital Camera Head and Nikon NSI-Elements Version 3.10 software. (B) Trafficking and homing of targeted T cells to BM. IHC staining on BM biopsies shows the presence of 19-28z+ T cells 5 and 6 weeks after infusion in patients ALL-1 and CLL-7, respectively (C) Trafficking and homing of targeted T cells to BM ≤ 8 weeks after infusion. ND indicates not done; (−) no positive 19-28z+ T cells found; (+) positive 19-28z+ T cells found occasionally, and (++) positive 19-28z+ T cells readily identifiable.

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