Expression profile of erythroid related genes in immature erythroid cells of Trim28 mutant mice. TMC [Trim28^flox/flox:Tg^Mx1Cre (flox/flox:Mx1Cre)] mutant or control [Trim28^flox/flox (flox/flox)] mice at ages 5 to 6 weeks were injected with poly(I:C) 5 times. Mice were analyzed 2 weeks after completing the poly(I:C) injections. CD71^hiTER119⁺ immature erythroid cells (closed circle) were isolated from control Trim28^flox/flox (flox/flox) mouse. CD71^hiTER119⁺ (open circle) and CD71^hiTER119^- (open triangle) immature erythroid populations were isolated from mutant Trim28^flox/flox:Tg^Mx1Cre (flox/flox:Mx1Cre) mouse. Cells were isolated from 3 animals of each genotype and were processed individually. RNA-Seq analysis was performed as described in “Methods.” Fragments per kilobase of exon per million mapped fragments for each animal are shown. Each circle represents an individual animal, whereas the black bars represent the averages. Gene names are shown at the top. Statistically significant changes in transcript expression analyzed in Cuffdiff analysis is depicted by a *. NS: not significant.