American Society of Hematology

Figure 6

$Figure 6. IR-induced long-term residual damage alters lymphopoiesis in a nonautonomous, Ink4a/arf-dependent manner. (A) Schematic of the experiment. Mice were exposed (+) or not (−) to IR and allowed to recover without transplantation. Eleven months later, mice were killed and BM cells were used for the transplantation of CD45.1 recipient mice. The proportion of donor-derived B cells (B220+ CD45.2+) was analyzed at different times in the blood before and after transplantation. n = 3-9 mice per group. (B-C) As described in Figure 5A, wild-type (WT) and Ink4a/arf-deficient (KO) CD45.2 mice exposed (+) or not (−) to 6 Gy of IR were transplanted 8 weeks later with unfractionated CD45.1 BM cells and allowed to reconstitute for 2 weeks before BM and spleen analysis of donor-derived CLP (B) and B220+ (C) cell counts. n = 3-5 mice per group. *P < .05, **P < .01, ***P < .001, and °P < .05 by Student t test (compared with primary mice).$

IR-induced long-term residual damage alters lymphopoiesis in a nonautonomous, Ink4a/arf-dependent manner. (A) Schematic of the experiment. Mice were exposed (+) or not (−) to IR and allowed to recover without transplantation. Eleven months later, mice were killed and BM cells were used for the transplantation of CD45.1 recipient mice. The proportion of donor-derived B cells (B220⁺ CD45.2⁺) was analyzed at different times in the blood before and after transplantation. n = 3-9 mice per group. (B-C) As described in Figure 5A, wild-type (WT) and Ink4a/arf-deficient (KO) CD45.2 mice exposed (+) or not (−) to 6 Gy of IR were transplanted 8 weeks later with unfractionated CD45.1 BM cells and allowed to reconstitute for 2 weeks before BM and spleen analysis of donor-derived CLP (B) and B220⁺ (C) cell counts. n = 3-5 mice per group. *P < .05, **P < .01, ***P < .001, and °P < .05 by Student t test (compared with primary mice).

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