Transplantability of Eµ-Myc/EZH2^Y641F lymphomas. (A) Kaplan-Meier survival analysis of mice transplanted with BM or spleen cells from 4 different Eµ-Myc/EZH2^Y641F donor mice with lymphoma. Transplants were injected into sublethally irradiated mice. Different donor mice exhibited different kinetics of lymphoma development in the secondary recipients independent of the cell source. (B) Southern blot analysis of IgH gene rearrangements for 2 different primary Eµ-Myc/EZH2^Y641F lymphoma mice and secondary recipients. The pattern of clonal rearrangements is maintained between primary and secondary lymphoma mice. (C) Kaplan-Meier survival analysis of mice transplanted with sorted BM cells from a secondary recipient mouse. Transplanted cell number and population are indicated. Only mice transplanted with unsorted BM cells and B220⁺IgM⁺ cells showed engraftment. Engraftment was measured by flow cytometry in the peripheral blood after staining for CD45.2.