Immunophenotypic characterization of the B-cell compartment in Eµ-Myc/EZH2^Y641F mice. (A) Wright-Giemsa stained cytospins from bone marrow of Eµ-Myc and Eµ-Myc/EZH2^Y641F mice. Images were taken at ×40. (B) Immunophenotypic analysis of bone marrow cells of Eµ-Myc (n = 14) or Eµ-Myc/EZH2^Y641F (n = 13) mice. (C) Immunophenotypic analysis of the bone marrow of Eµ-Myc (n = 8) and Eµ-Myc/EZH2^Y641F mice (n = 8) for B220 and IgM. Representative flow cytometric plots are shown and a quantification of the data with means and standard deviations. (D) Immunophenotypic analysis of B220⁺IgM⁺ cells in the bone marrow of Eµ-Myc (n = 8) and Eµ-Myc/EZH2^Y641F mice (n = 8) for expression of IgD. (E) Immunophenotypic analysis of B220⁺IgM⁺ cells in the bone marrow of Eµ-Myc (n = 8) and Eµ-Myc/EZH2^Y641F mice (n = 8) for expression of AA4.1. (F) Immunophenotypic analysis of the pro- (B220⁺IgM⁻CD43⁺) and pre-B-cell (B220⁺IgM⁻CD43⁻) compartment in the bone marrow of Eµ-Myc (n = 8) and Eµ-Myc/EZH2^Y641F mice (n = 8).