Differential expression of the cell-cycle regulator Cyclin D1 in EZH2^Y641F mice. (A) Quantitative PCR for different cell-cycle regulators (Cdkn1a, Cdkn1b, and Cdkn2a) known to be regulated by EZH2 and Cyclin D1 (Ccnd1) was performed on sorted B220⁺ cells from the spleen of Eµ-Myc (n = 3) and Eµ-Myc/EZH2^Y641F (n = 3) mice. Expression results are normalized to expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and expressed relative to the expression in B220 cells from a WT mouse. The P value was calculated using a Student t test. (B) Western blot analysis for the expression of Cyclin D1 in the spleen of WT, EZH2^Y641F, Eµ-Myc, and Eµ-Myc/EZH2^Y641F mice. Actin is shown as loading control.