Hemolytic anemia caused by loss of Trsp gene is partially compensated by Nrf2. (A) Peripheral blood counts of mice at 4 weeks after the final pI-pC injection. RBC, red blood cell; Hb, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; WBC, white blood cell; PLT, platelet. *P < .01 and **P < .001 compared with values obtained from Nrf2+/+:Trspfl/del mice. (B) Hct kinetics of mice treated with pI-pC. The day of the first pI-pC injection is depicted as 0. (C) Plasma indirect bilirubin levels were determined at 4 weeks after final pI-pC injection. Horizontal lines represent average values in each genotype (*P < .05; **P < .01). (D-K) Peripheral blood smears of mice at 4 weeks after the final pI-pC injection were stained with Wright-Giemsa (D-G) or new methylene blue (H-K). Arrows, white arrowheads, and black arrowheads indicate the poikilocytes, immature erythrocytes with basophilic cytoplasm, and erythrocytes with Howell-Jolly body, respectively.
