Erg regulates tubulin acetylation. HUVECs grown on gelatin-coated coverslips were transfected with either control-GB or Erg-specific GB for 48 hours. (A) Cells were labeled and visualized for Erg (red), tubulin (green), and nuclear marker TOPRO3 (blue). The microtubule network appears similar in control and Erg-deficient cells. Scale bar, 20 μm. (B) Cells were labeled and visualized for Erg (red), acetylated tubulin (green), and nuclear marker TOPRO3 (blue). Scale bar, 20 μm. In control cells, acetylated tubulin concentrates at the perinuclear region, as expected, whereas in Erg-deficient cells, acetylated tubulin is found throughout the cytoplasm.