The ADAP/SKAP55 module influences the intranodal motility of naive CD4⁺ T cells in vivo. (A) Equal cell numbers of Oregon Green–labeled WT and TAMRA-labeled ADAP^−/− CD4⁺ T cells (and vice versa) were adoptively transferred into WT recipients. After 24 hours, the paracortical T-cell zone of the popLN was imaged by intravital microscopy, and T cells were tracked semiautomatically using Imaris. Each dot represents the average track speed of an individual cell; and black bars, median values. Graph summarizes all tracked cells from the popLN imaging of 7 WT recipient mice. (B) Statistical comparison of the mean average track speed values of WT and ADAP^−/− CD4⁺ T cells, calculated separately for each imaging experiment (mean ± SEM; n = 6 for WT, n = 7 for ADAP^−/−). *P ≤ .05. (C) Mean displacement plot of WT (black squares) and ADAP^−/− (gray squares) CD4⁺ T cells. (D) Motility coefficient (mean ± SEM; n = 6 for WT, n = 7 for ADAP^−/−). ***P ≤ .005. (E) Track straightness (mean ± SEM; n = 6 for WT, n = 7 for ADAP^−/−). n.s. indicates not significant.