American Society of Hematology

Figure 5

$Figure 5. Two distinct pools of the ADAP/SKAP55 module are recruited to LFA-1. (A) Purified splenic WT and ADAP−/− T cells were left untreated or stimulated with CCL21 for the indicated time points. Plasma membrane fractions were prepared, lysed, and subjected to anti-CD18 immunoprecipitation. Subsequently, precipitates were analyzed by Western blotting, using the indicated antibodies. One representative experiment of 2 is shown. (B) Plasma membrane fractions as described in panel A were subjected to CD11a immunoprecipitation and then analyzed by Western blotting using the indicated antibodies. One representative experiment of 2 is shown. (C-D) Purified human T cells were left untreated or stimulated with CCL21 for the indicated time points. Lysates were incubated with GST-fusion proteins bound to glutathione-Sepharose beads. Precipitates were analyzed by Western blotting using the indicated antibodies. One representative experiment of 2 is shown.$

Two distinct pools of the ADAP/SKAP55 module are recruited to LFA-1. (A) Purified splenic WT and ADAP^−/− T cells were left untreated or stimulated with CCL21 for the indicated time points. Plasma membrane fractions were prepared, lysed, and subjected to anti-CD18 immunoprecipitation. Subsequently, precipitates were analyzed by Western blotting, using the indicated antibodies. One representative experiment of 2 is shown. (B) Plasma membrane fractions as described in panel A were subjected to CD11a immunoprecipitation and then analyzed by Western blotting using the indicated antibodies. One representative experiment of 2 is shown. (C-D) Purified human T cells were left untreated or stimulated with CCL21 for the indicated time points. Lysates were incubated with GST-fusion proteins bound to glutathione-Sepharose beads. Precipitates were analyzed by Western blotting using the indicated antibodies. One representative experiment of 2 is shown.

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