Phenotypic analysis of parenchymal and sinusoidal NK cells. Flow cytometric analysis of BM and LN NK cells isolated from WT mice injected with anti-CD45 and then stained in vitro with Abs for CD3, NK1.1, CD27, and CD11b. Numbers above bracketed lines or in quadrants indicate the percentage of cells in each area. (A) CD45 labeling of gated NK cells (NK1.1⁺ CD3⁻). (B-C) Analysis of CD45 staining in NK-cell subsets defined by CD27 and CD11b expression. (B) Representative FACS plot of CD27/CD11b expression in gated CD45⁻ (top panels) and CD45⁺ (bottom panels) NK cells. (C) Mean ± SD percentage of CD45⁻ (parenchymal, black bars) and CD45⁺ (sinusoidal, white bars) cells among CD11b^low, DP, and CD27^low NK cells calculated from the analysis of 10 mice.